cDNA相关论文
22. Proteomic Analysis of Differential Protein Expression in vero Cell with Antisense Blocking of Re
Objective: Recent studies have demonstrated that cells exposed to ionizing radiation or alkylating agents can develop pr......
鳜鱼（Chinese perch,Siniperca chuatsi）俗称鳜花鱼、季花鱼、桂花鱼、桂鱼、鯚鱼,属鲈形目,鮨科,鳜亚科,鳜属（Basilewsky）。鳜鱼广泛......
草原兔尾鼠是威胁新疆畜牧业发展的重要害鼠，其种群的相对过剩不利于草原生态系统的可持续发展。通过免疫不育方法控制鼠害已成为目......
采用RACE技术克隆获得虾夷扇贝热休克蛋白90(HSP90)基因cDNA全长序列,命名为MYHSP90.序列和结构分析表明:cDNA全长2524 bp,5\'-......
14-3-3蛋白可以与不同蛋白质结合从而参与多种磷酸化调节的信号通路,并在生物代谢、信号转导、细胞周期控制、凋亡、蛋白质运输、......
为研究小肽转运载体PepT1介导草鱼肠道对饲料蛋白消化吸收的分子机理,本文采用同源克隆和RACE技术首次克隆草鱼Pep T1基因的全长cD......
本研究首次从青虾(Macrobrachium nipponense)精巢中克隆得到Kazal型丝氨酸蛋白酶抑制因子(Kazal-type serine proteinase inhibit......
会议
本文采用RACE术获得了赤眼鳟(Squaliobarbus curriculus)TRIF(TIR-domain-containing molecule 1)基因的cDNA全长,命名为ScTRIF,Sc......
The diamondback moth,Plutella xylostella(Lepidoptera,Plutellidae),is a destructive pest of crucifers,and one of the most......
Leucocyte cell-derived chemotaxin 2 (LECT2) was first identified as a chemotactic factor and has been subsequently prove......
红笛鲷(Lutjanus sanguineus)俗称红鱼、红曹鱼，是我国南方沿海主要的经济鱼类之一。然而随着水产养殖业的迅猛发展，疾病频繁暴发，给......
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蠕虫埃斯特菌(Esteya vermicola)是首次报道寄生松材线虫(Bursaphelenchus xylophilus)的一种食线虫真菌，各种胞外酶尤其是丝氨酸蛋......
Molecular cloning of Japanese eel (Anguilla.japonica) TNF-α and characterization of its expression i
The full-length eDNA sequence and genomic structure of TNF-α (AjTNF-α) in Japanese eel (Anguilla.japonica) was identif......
为了了解萨罗罗非鱼水通道蛋白AQP3在渗透压调节中的作用.实验利用cDNA末端快速扩增法(RACE)克隆了萨罗罗非鱼Sarotherodon melano......
为了获得驯鹿β-防御素reBD-1全长cDNA序列,根据已获得的reBD-1 cDNA的已知序列设计一条序列特异性引物作为上游引物,反转录引物中......
鸡白洛克肉鸡(EE)和丝羽乌骨鸡(CC)两个纯种及其正交和反交组合EC和CE进行了产蛋高峰期(32周龄)卵巢组织的基因差异显示分析.结果:......
西尼罗病毒型脑炎是由属于黄病毒科(Flaviviridae),黄病毒属(Flavivirus)的西尼罗病毒(West Nile Virus,WNV)引起的病毒性疾病.WNV......
为了探讨尼罗罗非鱼(Oreochromis niloticus)新吉富品系、萨罗罗非鱼(Sarotherodon melanotheron)及其杂交子代(Oreochromis nilot......
采用同源克隆和末端快速扩增(RACE)方法,从牙鲆中克隆到1182bp的MHCⅡB全长cDNA序列.该序列包括741bp的开放阅读框(0RF),33bp的5末......
细胞凋亡是机体生长发育、细胞分化和病理状态中细胞自主性死亡的过程,由一系列有关的基因协调控制,这些基因被称为凋亡相关基因.......
Molecular Cloning,Expression Profiling and Function Analysis of Vitellogenin Gene in the Diamondback
The diamondback moth,Plutella xylostella(Lepidoptera,Plutellidae),is one of the most destructive pests attacking economi......
研究背景 家族性高胆固醇血症(FH)是一种严重的常染色体显性单基因遗传性疾病,其主要特点为血浆低密度脂蛋白胆固醇(LDLC)水平极度......
Analysis of gene expression profile in anther development of cytoplasmic male sterile Raphanus sativ
The anthers in flowers perform important functions in sexual reproduction.Seldom studies have been made to study anther ......
本研究采用cDNA基因芯片技术检测人卵巢癌组织中表达差异基因,为早期诊断卵巢癌及判断预后提供理想标志物。......
WilmsTumor(肾胚瘤)是一种较为常见的儿科恶性实体肿瘤,加5﹪～25﹪的肾胚瘤患者发生WT-1基因的改变.实验中建立了肾胚瘤的裸鼠移植瘤模......
以Molt3、Hut78和5D1细胞为实验材料，建立了适用于5'端序列不清的TCR cDNA扩增的锚连接PCR方法，经Southern杂交鉴定，证实本方法具有......
应用基因重组技术，将人肿瘤坏死因子α（hTNFα）cDNA去前导序列并修饰后，克隆到表达载体pBV220的PRPL启动子下游，并转化到DH5α菌后，经发......
用带脊髓灰质炎病毒（Polio-virus）Ⅰ型Mahoney株的cDNA质粒转染HeLa细胞后，产生完整病毒颗粒。实验采用了DEAE-Dextran转染法，将环状pS......
呼吸道合胞病毒（RSV）是婴幼儿严重下呼吸道感染的重要病因，也是成人急性呼吸道感染的病因之一。近年来，有关RSV感染的基因诊断技术已逐......
目的应用cDNA分子克隆测序方法，鉴定中国南方汉族1个KIR3DL3新等位基因。方法采集1例KIR3DL3基因测序结果异常的无偿志愿捐血者的外......
为建立人血小板cDNA文库，作者用人新鲜血小板悬液纯化血小板，加入变性液作匀浆，经苯酚、氯仿、异丙醇等抽提RNA，以所得RNA为模板合成cD......
大疱性类天疱疮(BP)存在两个自身抗原:BPAGl(220000～240000)和BPAG2(160000～180000),二者为不同的基因产物。为了研究BP的分子生物学......
Molecular cloning,expression profiling and function analysis of vitellogenin gene in the diamondback
The diamondback moth,Plutella xylostella(Lepidoptera,Plutellidae),is one of the most destructive pests attacking eco......
Molecular cloning, expression profiling and function analysis of vitellogenin gene in the diamondbac
The diamondback moth, Plutella xylostella (Lepidoptera, Plutellidae), is one of the most destructive pests attacking......
本文介绍了首次克隆出鹿FSH-α-亚基基因,并将它与多种动物的同类基因进行了比较.此基因编码区由360个碱基,120个氨基酸组成.......
功能基因组学的开展为基因结构、功能及其表达产物特性的研究提出了更高要求,完整的基因信息是必需的,因此基因全长的克隆成为研究......
采用cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE),在本课题组已获得的驯鹿ghrelin cDNA的部分片段的基础上进行......
目的 获得ＳＴ１３基因ｃＤＮＡ５＇端的未知序列。方法 应用套式ＰＣＲ方法直接从ｃＤＮＡ文库中扩增该基因的５＇端序列，然后将扩增的片段克隆到ｐＧＥＭ－５．ｅａｓｙ载体，并进行序列分析。结......
雄蕊的发展在在更高的植物的性繁殖期间是一个重要阶段。学习主要或甚至明确地在雄蕊表示了的基因将在 undestanding 帮助雄蕊开发......
A new method designated cDNA array was developed by hybridization of quantitatively arrayed DNA samples isolated randoml......
Based on the cDNA fragment sequence of vernalization-related gene verc203 cloned by differential screening in our lab, t......
Cloning and sequencing of cDNA fragment of gene of wheat (Triticum aestivum. L) induced by dehydrati
cDNA fragment of the gene (dehydration induced, di1) of wheat (Triticum aestivum. L) induced by 30% PEG-6000 (-1.13 MPa)......
Molecular cloning and expression analysis of a novel human cDNA fragment encoding a putative Ser/Thr
A novel full-length cDNA encoding a putative serine/threonine kinase has been isolated from a human testis cDNA library.......
cDNA 克隆， pS4，从在长度从大约 1.0 公里的米饭花药准备的一个 cDNA 图书馆被孤立。DNA 顺序分析和数据库搜索证明 cDNA 编码对真核......
The mbl (musdeblind) gene of Drosophila encodes a nuclear protein which contains two Cys3His motifs. The mutation of mbl......
Protein kinase B (PKB) is a member of the second messenger-regulated subfamily of protein kinases, and plays a key role ......
